Rohan Fernandes, PhD joined the Institute as an Assistant Professor in the Bioengineering Initiative in December 2011. Dr. Fernandes’ research interests include developing multifunctional nanoparticles for theranostics (i.e. simultaneous therapy and diagnostics) of pediatric cancers and inflammatory diseases.
Dr. Fernandes’ current research focuses on repurposing Prussian blue (an ancient dye) as an MRI contrast agent, a disease site-specific fluorescent “paint”, a drug delivery agent, and as an agent for laser-induced photothermal ablation of tumors. Dr. Fernandes’ group is currently testing these novel capabilities of Prussian blue in preclinical models of pediatric cancers and inflammatory diseases. His group closely collaborates with investigators at Children’s, Howard University, and the University of Maryland. Dr. Fernandes completed his PhD in Bioengineering from the University of Maryland in 2008. After his PhD, Dr. Fernandes completed his Postdoctoral Fellowship from Johns Hopkins University in 2011.
1. Fernandes R, Wu LQ, Chen TH, Yi HM, Rubloff GW, Ghodssi R, Bentley WE and Payne GF, “Electrochemically induced deposition of a polysaccharide hydrogel onto a patterned surface,” Langmuir, Vol. 19, No. 10, 4058-4062, 2003.
2. Fernandes R, Yi HM, Wu LQ, Rubloff GW, Ghodssi R, Bentley WE and Payne GF. “Thermo-biolithography: a technique for patterning nucleic acids and proteins,” Langmuir, Vol. 20, No. 3, 906-913, 2004.
3. Fernandes R, Tsao CY, Hashimoto Y, Wang L, Wood TK, Payne GF and Bentley WE. “Magnetic nanofactories: localized synthesis and delivery of quorum-sensing signaling molecule autoinducer-2 to bacterial cell surfaces,” Metabolic Engineering, Vol. 9, No. 2, 228-239, 2007.
4. Fernandes R and Bentley WE. “AI-2 biosynthesis module in a magnetic nanofactory alters bacterial response via localized synthesis and delivery,” Biotechnology & Bioengineering, Vol. 102, No. 2, 390-399, 2009.
5. Wu HC, Shi XW, Tsao C, Lewandowski AT, Fernandes R, Hung CW, DeShong P, Kobatake E, Valdes JJ, Payne GF and Bentley WE. “Biofabrication of antibodies and antigens via IgG-binding domain engineered with activatable pro-tag,” Biotechnology & Bioengineering, Vol. 103, No. 2, 231-240, 2009.
6. Koev ST, Fernandes R, Bentley WE and Ghodssi R. “A cantilever sensor with integrated optical readout for detection of enzymatically produced homocysteine,” IEEE Transactions on Biomedical Circuits and Systems, Vol. 3, No. 6, 415-423, 2009.
7. Fernandes R and Gracias DH. “Toward a miniaturized mechanical surgeon,” Materials Today, Vol. 12, No. 10, 14-20, 2009.
8. Fernandes R, Roy V, Wu HC and Bentley WE. “Engineered biological nanofactories trigger quorum sensing response in targeted bacteria,” Nature Nanotechnology, Vol. 5, No. 3, 213-217, 2010.
9. Roy V, Fernandes R, Tsao C and Bentley WE. “Cross species quorum quenching using a native AI-2 processing enzyme,” ACS Chemical Biology, Vol. 5, No. 2, 223-232, 2010.
10. Fernandes R, Luo XL, Tsao C, Payne GF, Ghodssi R, Rubloff GW and Bentley WE. “Biological nanofactories facilitate spatially selective capture and manipulation of quorum sensing bacteria in a bioMEMS device,” Lab on a Chip, Vo. 10, No. 9, 1128-1143, 2010 (Featured on the inside front cover).
11. James T, Cho J-H, Fernandes R, Randhawa JS and Gracias DH. “A one-step etching method to produce gold nanoparticle coated silicon microwells and microchannels,” Analytical & Bioanalytical Chemistry, Vol. 398, No. 7-8, 2949-2954, 2010.
12. Hebert CG, Gupta A, Fernandes R, Tsao CY, Valdes JJ and Bentley WE. “Biological nanofactories target and activate epithelial cell surfaces for modulating bacterial quorum sensing and interspecies signaling,” ACS Nano, Vol. 4, No. 11, 6923-6931, 2010.
13. Azam A, Laflin KE, Jamal M, Fernandes R and Gracias DH. “Self-folding micropatterned polymeric containers,” Biomedical Microdevices, Vol. 13, No. 1, 51-58, 2011.
14. Fernandes R, Zuniga M, Sassine FR, Karakoy M and Gracias DH. “Enabling cargo-carrying bacteria via surface attachment and triggered release,” Small, Vol. 7, No. 5, 588-592, 2011.
15. Fernandes R and Gracias DH. “Self-folding polymeric containers for encapsulation and delivery of drugs,” Advanced Drug Delivery Reviews, Vol. 64, No. 14, 1579-1589, 2012.
16. Gupta A, Terrell JL, Fernandes R, Dowling MB, Payne GF, Raghavan SR and Bentley WE. ““Sense and Respond” Activity to Chitosan-Alginate Capsules To Manipulate Bacterial Quorum Sensing,” Biotechnology & Bioengineering, Vol. 110, No. 2, 552-562, 2013.
17. Jamal M, Kadam SS, Xiao R, Jivan F, Onn TM, Fernandes R, Nguyen TD and Gracias DH. “Bio-origami hydrogel scaffolds comprised of photocrosslinked PEG bilayers,” Advanced Healthcare Materials, Vol. 2, No. 8, 1142-1150, 2013.
18. Dumont MF, Hoffman HA, Yoon PRS, Conklin LS, Saha SR, Paglione J, Swe RW and Fernandes R*. “Biofunctionalized Gadolinium-Containing Prussian Blue Nanoparticles as Multimodal Molecular Imaging Agents,” Bioconjugate Chemistry, Vol. 25, No. 1, 129-137, 2014.
19. Dumont MF, Yadavilli S, Sze RW, Nazarian J and Fernandes R*. “Manganese-containing Prussian blue nanoparticles for imaging of pediatric brain tumors,” International Journal of Nanomedicine, DOI: 10.2147/IJN.S63472, 2014.
*indicates senior/corresponding author
1. Koev ST, Fernandes R, Bentley WE and Ghodssi R. “A microcantilever sensor with integrated optical readout for antimicrobial drug discovery,” 15th International Conference on Solid-State Sensors, Actuators, and Microsystems, Denver, CO, 2009.
2. Fernandes R, James T, Zuniga MC, Li H, Ngan S and Gracias DH. “Assembling backpacking bacteria for diagnostics and therapeutics,” 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS), Seattle, WA, 2011.
1. Payne GF, Rubloff GW, Yi HM, Fernandes R, Wu LQ, Ghodssi R, Bentley WE. “Biolithographical deposition and materials and devices formed therefrom,” U.S. Patent 7,820,227.
2. Payne GF, Wu LQ, Ghodssi R, Bentley WE, Rubloff GW, Yi HM, Fernandes R, Chen TH, Small DA. “Controlled electrochemical deposition of polysaccharide films and hydrogels, and materials formed therefrom,” U.S. Patent 7,883,615.
1. Fernandes R, Dumont MF, Sze RW, Conklin LS, Jaiswal JK, Hoffman HA. “Prussian-blue inspired constructs for multimodal imaging and therapy.” US Patent Application No. 14/213380